Product Description
Silica gel for column chromatography, 300-400 mesh medium particle size.
SiO2 300-400 mesh (75-38µm particle size), pH 6-7, pore size 60Å
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Lot Experimental Data
Density (loose): 0.38g/cm³
Density (well-packed): 0.45g/cm³
Column volume: 42mL/20g
Normalized flow rate (gravity): 4.5mL/min
Normalized flow rate (2psi): 31mL/min
While this silica will enable a fast flow, there is no need for a long column. A short, thick column will generally be better, as long as sample is loaded as a thin layer and column is homogenously packed. Because if a TLC plate of 6-7cm long can separate your compound, so should and can a column of ~10ish cm. A long column will only cause unnecessary peak broadening and if using fine-size silica, high back-pressure.
We care for you. No one should be exposed to silica dust. What causes the most harm may not be the most toxic, but the most common reagent due to exposure amounts. With our specially designed and packed SilicaDispense(TM) bottles, say goodbye to the hassle and hazards of handling silica powder in your lab work. Easily twist open to uncap and squeeze out the silica directly into your column or final apparatus. You do not need to transfer from a large container to a secondary container. You do not need any tools or spatulas, while the SiliDispense(TM) bottles will fit in most lab spatulas if for any reason a spatula is to be used. Bottle size designed for benchtop storage and direct use. The sturdy, empty bottles can be reused for solvent storage and dispensing indefinitely.
See photos and video demonstration for this great convenience and safe health feature.
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The following is an actual separation experiment performed with this silica:
20g of silica (300-400mesh) was added to a cotton-blocked glass chromatography column with I.D. 2.6cm (silica height ~10cm). Silica column was then wetted and packed using AAdvance’s in situ wet pack method. Column volume (lot) was 40mL. 0.2g dimethyl maleate (d.m.m.) and 0.2g dimethyl fumerate (d.m.f.) were loaded onto column as a suspension in 5% ethyl acetate in hexanes (4mL). Then, 10% ethyl acetate in hexanes was added as eluting solvent to start separation. Elution was conducted with a gravity flow and no extra pressure was applied. Solvent was collected with test tubes with volumetric marks. The first fraction (d.m.f.) started eluting out at 70mL and finished eluting by 105mL. The second fraction (d.m.m.) started coming out at 125mL, and at the same time eluting solvent was replenished with 25% ethyl acetate in hexanes instead. Eluting finished at around 160mL. See separation results by TLC spotting. This is a challenging separation, not only because of the compounds’ closeness in Rf and polarity, but also due to the fact that d.m.f. dissolves poorly in hexanes or 5% ethyl acetate in hexanes, causing peak broadening.
SiO2 300-400 mesh (75-38µm particle size), pH 6-7, pore size 60Å
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Lot Experimental Data
Density (loose): 0.38g/cm³
Density (well-packed): 0.45g/cm³
Column volume: 42mL/20g
Normalized flow rate (gravity): 4.5mL/min
Normalized flow rate (2psi): 31mL/min
While this silica will enable a fast flow, there is no need for a long column. A short, thick column will generally be better, as long as sample is loaded as a thin layer and column is homogenously packed. Because if a TLC plate of 6-7cm long can separate your compound, so should and can a column of ~10ish cm. A long column will only cause unnecessary peak broadening and if using fine-size silica, high back-pressure.
We care for you. No one should be exposed to silica dust. What causes the most harm may not be the most toxic, but the most common reagent due to exposure amounts. With our specially designed and packed SilicaDispense(TM) bottles, say goodbye to the hassle and hazards of handling silica powder in your lab work. Easily twist open to uncap and squeeze out the silica directly into your column or final apparatus. You do not need to transfer from a large container to a secondary container. You do not need any tools or spatulas, while the SiliDispense(TM) bottles will fit in most lab spatulas if for any reason a spatula is to be used. Bottle size designed for benchtop storage and direct use. The sturdy, empty bottles can be reused for solvent storage and dispensing indefinitely.
See photos and video demonstration for this great convenience and safe health feature.
----------------------------------------
The following is an actual separation experiment performed with this silica:
20g of silica (300-400mesh) was added to a cotton-blocked glass chromatography column with I.D. 2.6cm (silica height ~10cm). Silica column was then wetted and packed using AAdvance’s in situ wet pack method. Column volume (lot) was 40mL. 0.2g dimethyl maleate (d.m.m.) and 0.2g dimethyl fumerate (d.m.f.) were loaded onto column as a suspension in 5% ethyl acetate in hexanes (4mL). Then, 10% ethyl acetate in hexanes was added as eluting solvent to start separation. Elution was conducted with a gravity flow and no extra pressure was applied. Solvent was collected with test tubes with volumetric marks. The first fraction (d.m.f.) started eluting out at 70mL and finished eluting by 105mL. The second fraction (d.m.m.) started coming out at 125mL, and at the same time eluting solvent was replenished with 25% ethyl acetate in hexanes instead. Eluting finished at around 160mL. See separation results by TLC spotting. This is a challenging separation, not only because of the compounds’ closeness in Rf and polarity, but also due to the fact that d.m.f. dissolves poorly in hexanes or 5% ethyl acetate in hexanes, causing peak broadening.
Product Information
- Silica gel for column chromatography, 300-400 mesh medium particle size. SiO2 300-400 mesh (50-38µm particle size), pH 6-7, pore size 60Å
- While this silica will enable a fast flow, there is no need for a long column. A short, thick column will generally be better, as long as sample is loaded as a thin layer and column is homogenously packed.
- With our specially designed and packed SilicaDispense(TM) bottles, say goodbye to the hassle and hazards of handling silica powder in your lab work.
- You do not need to transfer from a large container to a secondary container. Easily twist open to uncap and squeeze out the silica directly into your column or final apparatus.
- You do not need any tools or spatulas, while the SiliDispense(TM) bottles will fit in most lab spatulas if for any reason a spatula is to be used. Bottle size designed for benchtop storage and direct use.
- Also available in 200-300 mesh, 500-800 mesh. Please visit our storefront for more information.
